Three deer, A, B, and C, are grazing in a field. Deer B located 61 m from dear A at an angle of 54° north of west. Deer C is located 76° north of east relative to dear A. The distance between deer B and C is 96 m. What is the distance between deer A and C?
· 11. How much time will it take a car travelling at 88 km/hr (55 mi/hr) to travel 500km?
· During a race, a runner runs at a speed of 6 m/s. 2 seconds later, she is running at a speed of 10 m/s. What is the runner’s acceleration?
Hi dr ;really I need help as soon as passible ..iam in master program ,medical education master.and I have assignment ,and I confused about it ,,even am not understand it the assignment is ;
you are a member of a course committee in the department of internal medicine .which is charged with the task of integration the topic of ethics into the third year medicine rotation .your committee has been given six blocks of two hours over a 12 week period .you wonder how to make material engaging understandable and useful to the student..
hree equal point charges, each with charge 1.15 mu C, are placed at the vertices of an equilateral triangle whose sides are of length 0.500 m. What is the electric potential energy U of the system? (Take as zero the potential energy of the three charges when they are infinitely far apart.)
a large wooden turntable in the shape of a flat uniform disk has a radius of 2.00 m and a total mass of 120 kg. the turntable is initially rotating at 3.00 rad/s about a vertical axis through its center. suddenly, a 70.0 kg parachutist makes a soft landing on the turntable at a point near the outer edge. Find the angular speed of the turntable after the parachutist lands. (Assume parachutist treated as particle)
GPA – SMART Goals Worksheet
Program:
Specific: You are half way through your program, what exactly do I want accomplish? Why do I want to accomplish this?
Measurable: How will you measure your progress? (ie What will you use to determine if you have reached any milestone to this goal?)
Achievable: How can my goal(s) be accomplished? (Is achieving this realistic
6.- Your friend Silvester wishes to subclone a 1.7 kb DNA fragment into the pCR 2.1 TOPO plasmid vector. His goal is to amplify the desired fragment by PCR. At the end of the PCR cycling reaction, he immediately adds 4 μL of PCR reaction, (the maximum of DNA allowable) to the ligation mix. He did the transformation and plated it onto solid media containing the right amount of X-gal and ampicillin.
He picked a single white colony, inoculated it into liquid LB media with ampicillin and grew it up overnight. The next day he purified the plasmid DNA from the colony and sent it for sequencing. He prepared two tubes of DNA, one sample of plasmid will be sequenced using the M13 -20 primer to sequence the bottom strand. The second DNA sample will be sequenced with the M13 Reverse primer to sequence the upper strand. Silvester got the sequence back and the chromatograms were very good. When he BLAST’ed the sequences using NCBI he realized that he had cloned a PCR product that was only 800 bp. He was upset because he felt that since he’d used blue-white selection and chose a white colony that he should have isolated the fragment he was interested in. He is confused and does not understand his results.
(i) Why was Silvester able to conclude with confidence that the PCR product he submitted for sequencing was only 800 bp? (10 pts).
(ii) Explain why a 800 bp sequence was ligated into the pCR 2.1 TOPO vector although his aim was to amplify a larger PCR product (5 pts).
(iii) Give him some recommendations that would help to him to select the right clones for sequencing in the future (15 pts).
a solution consisting of isobutyl bromide and isobutyl chloride is found to have a refractive index of 1.3931 at 20 degrees celsius. The refractive indices at 20 degrees celsius of isobutyl bromide and isobutyl chloride are 1.4368 and 1.3785. determine the molar composition in percent of the mixture by assuming a linear relation between the refractive index and the molar composition of the mixture?
The amount of NH4+ ions present as NH4NO3 in a sample of fertilizer is to be determined. Direct titration of NH4+ would not reveal an accurate result. Instead, NH4NO3 is reacted with Xs NaOH and this solution is then titrated with HCl in order to determine the amount of -OH required to neutralize all the NH4+ ions.
1.50 g of fertilizer is transferred carefully into a 100 mL volumetric flask and made up to the mark, using distilled water. Aliquots of 10 mL of this solution are then added to conical flasks that also contain 40 mL of 0.210 M NaOH.
These solutions are then titrated with a standard solution of 0.26 M HCl (average titre is 28.80 cm3).
Calculate the number of moles of NH4+ ions in the 100 mL volumetric flask and the % by mass NH4NO3 in the fertilizer
Nursingessaytutors.com an online service provider ensuring the deliverance of unparalleled services to college students. You can rely on our professional service to achieve your academic goals.
Hi there! Click one of our representatives below and we will get back to you as soon as possible.